Salt-Tolerant DNA Polymerases

Methods of performing DNA synthesis using salt tolerant versions of DNA polymerases derived from Bacillus phage phi29 are described. Such polymerases can function in salt solutions with concentrations as high as 25% wt/volume of salt (KCl, NaCl or othe…

Methods of performing DNA synthesis using salt-tolerant versions of DNA polymerases derived from Bacillus phage phi29 are described. Such polymerases can function in salt solutions with concentrations as high as 25% wt/volume of salt (KCl, NaCl, or other monovalent salts).

Abstract

Various scientific and industrial applications exist in which it would be advantageous to use a DNA polymerase that functions efficiently at high salt concentrations. In sequencing, GC compressions can be resolved by using high salt concentrations. In nanopore sequencing, high salt con­centration boosts the signal-to-noise ratio for ionic-current ­based nanopore measurements.

Website

https://techtransfer.universityofcalifornia.edu/NCD/32798.html?utm_source=AUTMGTP&utm_medium=webpage&utm_term=ncdid_32798&utm_campaign=TechWebsites

Contact Information

Name: Jeff Jackson

Email: jjackso6@ucsc.edu

Phone: (831) 459-3976