SageSeq for Discovering Large Deletions Produced by Genome Editing Protocols

Abstract:

Gene editing typically involves the use of a targeted nuclease to induce double-strand

DNA breaks (DSBs) at specific genomic sites. DSBs are then repaired by one of two cellular mechanisms: homology-directed repair (HDR) which uses a DNA template to repair the DSB, while nonhomologous end-joining (NHEJ) directly repairs the DSB but frequently creates an insertion or deletion mutation (indel) at the DSB site. Because HDR is usually less efficient than NHEJ, even protocols that use a DNA template to edit the region around a DSB result in a large proportion of repaired alleles containing an indel. Current methods for determining the genotypes produced by genome editing involve Polymerase Chain Reaction (PCR) amplification of DNA fragments followed by deep sequencing. These methods have limitations and do not inform the full extent of the induced indel landscape. Accordingly, new and improved methods for discovering indels induced by a genome editing process are needed.

The researchers discovered methods of generating a catalog of mutations induced by a genome editing protocol that can provide an unbiased, full landscape of mutations including large indels, and/or remote indels that are distant from the targeted editing site and can identify a DNA variant un-intendedly induced by a genome editing protocol.

Website:

https://techtransfer.universityofcalifornia.edu/NCD/32330.html?utm_source=AUTMGTP&utm_medium=webpage&utm_term=ncdid_32330&utm_campaign=TechWebsites

Advantages:

  • Precise gene editing

Potential Applications:

  • gene editing
  • research tool

Additional Information:

Tech ID/UC Case

32330/2021-129-0

Related Cases

2021-129-0

Contact Information:

Name: Terri Sale

Title :

Department :

Email: terri.sale@berkeley.edu

Phone: 510-643-4219

Address :