Rapid Nano-Flow Cytometry for Virus Detection

A novel nano-flow cytometry approach that can detect very small bio-particles such as viruses.

A novel nano-flow cytometry approach that can detect very small bio-particles such as viruses.

Flow cytometry is a general method for rapidly analyzing large numbers of cells individually using light-scattering, fluorescence, and absorbance measurements. However, the size measurements are highly influenced by the refraction index, and thus practically limited to larger-sized particles. For very small particles, the scattering light intensity drops quickly and thus it becomes rather difficult to determine the size of the virus and other small important biological particles. Here we present a promising flow cytometry approach proposed by a group of researchers led by Prof. Tsumoru Shintake. The novel approach is based on using an interference fringe to rapidly detect nano-sized biological particles such as viruses.

This novel flow cytometry technology is based on a system where two laser beams overlap, creating interference fringe (like Young's experiment), whose pitch becomes a smaller wavelength. As a result of the interference fringe, this novel approach can detect scattering light from particles and measure modulation depth (Fourier component of particle shape), which gives particle size for smaller viruses and other biological particles.

Applications

  • Biological analytics
  • Virus detection

Advantages

  • Nanoparticle detection range
  • Absolute size measurement
  • Rapid analysis