Photopatterning Spatially Controlled Chemically Functionalized Hydrogels

Researchers at the University of California, Santa Barbara have developed a Diels–Alder-based photopatterning platform that is capable of spatially controlled chemical functionalization of hydrogel systems. The platform achieves the precise patte…

Researchers at the University of California, Santa Barbara have developed a Diels–Alder-based photopatterning platform that is capable of spatially controlled chemical functionalization of hydrogel systems. The platform achieves the precise patterning of biochemical cues within hydrogels by exposing cyclopentadienone–norbornadiene (CPD–NBD) adducts to a specific wavelength of light. The adducts undergo light-induced decarbonylation and subsequent cleavage, unveiling thermodynamically stable yet highly reactive cyclopentadiene for post-irradiation reaction with commercially available maleimides. Unlike existing photopatterning platforms, this strategy does not involve radical propagating photo click reactions, making it compatible with natural protein-based hydrogels. This platform for preparing biomimetic microenvironments has the potential to facilitate new discoveries concerning cell fate decisions with direct application to cell-based therapeutics.

Website

https://techtransfer.universityofcalifornia.edu/NCD/32834.html?utm_source=AUTMGTP&utm_medium=webpage&utm_term=ncdid_32834&utm_campaign=TechWebsites

Advantages

  • Spatially controlled hydrogel functionalization mimics heterogeneity of natural tissues
  • Avoids propagating radical mechanism, improving compatibility with ubiquitous extracellular matrix (ECM) polymers such as collagen, laminin, and Matrigel
  • Compatible with commercially available maleimide-bearing fluorescent probes and biomolecules

Potential Applications

  • Cell-based therapeutics
  • Tissue engineering
  • Textured in vitro meat production
  • Cellular research

Contact Information

Name: Mary Raven

Email: raven@tia.ucsb.edu