One-Pot Multiplex Gene Synthesis (Option-Agilent)

Abstract

Gene synthesis is an invaluable tool in the pharmaceutical and synthetic biology industries, allowing researchers to create artificial genes of any desired sequence. The use of gene synthesis in the industry has been limited due to the high cost, low throughput, and poor automatability of synthesis technologies.  Scientists at UC Berkeley have developed a new method of gene synthesis that uses microarray fabrication of DNA strands, which overcomes these limitations. Their technology employs commonly available materials and tools, resulting in significantly reduced costs and straightforward operation. Furthermore, their technology allows for automated, high throughput of many genes or gene libraries in parallel, reducing labor and increasing productivity. This technology has many applications in synthetic biology, including protein engineering, vaccine development, and gene therapy. 

Website

https://techtransfer.universityofcalifornia.edu/NCD/23291.html?utm_source=AUTMGTP&utm_medium=webpage&utm_term=ncdid_23291&utm_campaign=TechWebsites

Advantages

  • High throughput: can synthesize many genes in parallel
  • Reduced cost of synthesis
  • Automated process; reduced labor
  • Can produce either a set of specific genes or a library of genes
  • Uses commonly-available materials

Potential Applications

  • Gene synthesis for commercial and research life science investigations
  • Molecular and protein engineering­­
  • Vaccine development­
  • Gene therapy

Contact Information

Name: Terri Sale

Email: terri.sale@berkeley.edu

Phone: 510-643-4219