Novel Assay Using Azide-Capture Agents

Prof. Min Xue from the University of California, Riverside, and Prof. Wei Wei from the Institute for Systems Biology have developed materials and methods to detect and measure FA uptake alone or simultaneously with protein detection in multiplex down to single-cell resolution. FA analogs with an azide functional group mimic natural FAs. Specially designed small polymers are used to efficiently assay the FA analogs and produce fluorescent or chemical signals upon binding. The technology is compatible with protein analysis and generally applicable to other metabolites and proteins.

Fig 1: Schematic of the UCR-ISB method for detecting fatty acid uptake from single cells.

Website

https://techtransfer.universityofcalifornia.edu/NCD/32824.html?utm_source=AUTMGTP&utm_medium=webpage&utm_term=ncdid_32824&utm_campaign=TechWebsites

Potential Applications

  • To assay for azide-modified molecules, such as FAs, at bulk or single-cell level.
  • To analyze cellular metabolism by integrating metabolic and proteomic readouts.

Contact Information

Name: Grace Yee

Email: grace.yee@ucr.edu

Phone: 951-827-2212