Generate Total Internal Reflection (TIRF) Illumination for High-Resolution Quantitative Fluorescence Microscopy

System for creating uniform, artifact-free TIRF illumination over a large field of view Reshapes the excitation source light into a ring-shaped beam using a fiber bundleProvides high power throughput without complex components or moving parts
  • System for creating uniform, artifact-free TIRF illumination over a large field of view
  • Reshapes the excitation source light into a ring-shaped beam using a fiber bundle
  • Provides high power throughput without complex components or moving parts

Abstract:

The University of Central Florida invention is an imaging method and system that reshapes Total Internal Reflection Fluorescence (TIRF) excitation light into a ring-shaped beam, which is conjugated to the back focal plane of an objective. The output end of the device comprises multiple multi-mode fibers arranged around a spacer to form a single ring. The device enables TIRF illumination with incoherent or coherent sources, provided that the coherence of the source is degraded before inputting the annular bundle. The device requires no moving parts, provides high excitation power throughput, and can generate uniform, artifact-free TIRF illumination over a large field of view.

Advantages:

  • Useful in quantitative fluorescence microscopy for imaging surface features with a high signal-to-background ratio
  • Compatible with coherent or incoherent light sources
  • High excitation power throughput with no complex components, moving parts
  • Produces no shadowing artifacts during subcellular imaging and provides a large field of view

Potential Applications:

  • Live-cell imaging on biomarkers near the surface
  • Single-molecule fluorescence imaging
  • High-throughput, large field-of-view cellular fluorescence imaging
  • Super-resolution fluorescence imaging

Contact Information:

  • Name : John Miner
  • Title :
  • Department :
  • Email : John.Miner@ucf.edu
  • Phone : 407.882.1136
  • Address :