UCLA researchers have developed a method to identify hundreds to thousands of regulatory regions from MPRAs. Their approach uses a combination of experimental and computational steps and is called systematic high-resolution activation and repression profiling with reporter tiling using MPRA (SHARP-MPRA). They tested their technology in two cell lines expressing 4.6 million nucleotides targeting 15000 putative regulatory regions and identified known regulatory genes. Their method also distinguished between known activating and repressing genes providing previously unknown information about the genetic motifs.
Abstract
UCLA researchers in the Department of Biological Chemistry have developed a method to screen hundreds to thousands of genes to identify their regulatory functions.
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Advantages
- Coupled with MRSA, an established method that is already in use
- Can distinguish between activating and repressing genetic motifs, ‘dual-role’ genes and motifs that attenuate active chromatin states
Potential Applications
Identifying activating and repressing genetic elements
Contact Information
Name: UCLA Technology Development Group
Email: ncd@tdg.ucla.edu
Phone: 310.794.0558