SELection of Modified Aptamers with fluorinated glycoRNA aptamers

  • The invention describe a facile synthetic route to Man3 and Man4 derivatives with a non-reducing-terminal sulfur linkage that is highly resistant to enzymatic degradation
  • This invention makes possible and confirms the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase

Advantages

  • The synthetic route introduced by the invention can be readily amenable to preparation of higher branched stabilized oligomannose analogs
  • Sulfur-substituted sugar makes possible to produce HIV vaccine that is highly resistant to enzymatic degradation.

Potential Applications

This invention demonstrates a facile synthetic route to Man3 and Man4 derivatives with a non-reducing-terminal sulfur linkage that is highly resistant to enzymatic degradation. These derivatives are recognized by an HIV antibody, 2G12, through contacts that are similar to those it makes with the natural oligomannose structure. This synthetic strategy should be readily amenable to preparation of higher branched stabilized oligomannose analogs, suitable for immunogenicity studies in the near future.