Methods of performing DNA synthesis using salt-tolerant versions of DNA polymerases derived from Bacillus phage phi29 are described. Such polymerases can function in salt solutions with concentrations as high as 25% wt/volume of salt (KCl, NaCl, or other monovalent salts).
Various scientific and industrial applications exist in which it would be advantageous to use a DNA polymerase that functions efficiently at high salt concentrations. In sequencing, GC compressions can be resolved by using high salt concentrations. In nanopore sequencing, high salt concentration boosts the signal-to-noise ratio for ionic-current based nanopore measurements.
Name: Jeff Jackson
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