Imaging and Tracking mRNA in Live Mammalian Cells via Fluorogenic Photoaffinity Labeling

Application
An improved method for labeling and imagine RNA in live cells.

Key Benefits
Low background signal.
Covalent binding suitable for time-resolved investigations.
Photoactivatable for temporal control.
Market Summary
Ribonucleic acid (RNA) is an essential molecule involved in transmitting information from DNA through the process of transcription. Thus, labeling and imaging RNA is important. Current RNA labeling approaches suffer from high background, poor durability of signal, and the need for several copies of RNA aptamer probes. Researchers at Emory University have developed a photoaffinity approach to RNA imaging and tracking that has low background signal, allows tracking of RNAs labeled during a specific time window, and is photoactivatable, among other benefits. The nucleic acid labeling market was valued at $1.71 billion in 2019 and is project to grow to $3.28 billion by 2027 at a CAGR of 8.4% (Reports and Data, July 2020).

Technical Summary
Researchers developed a novel method for improved labeling and imaging RNA in live cells using a photoaffinity approach. In this method the Malachite Green Aptamer (MGA) ligand is functionalized with a photoactivatable reaction for irradiation with UV light results in covalent attachment to the RNA of interest. Furthermore, the researchers incorporated a photoaffinity linker onto the MGA and fused said MGA to a specific mRNA reporter of interest. This demonstrated significantly improved sensitivity for fixed cells and live cells imaging of mRNA.

Developmental Stage
Prototype tested.

Website

https://emoryott.technologypublisher.com/techcase/20092

Contact Information

TTO Home Page: https://emoryott.technologypublisher.com

Name: Sat Balachander

Title: Licensing Associate

Email: sbalac5@emory.edu

Phone: (404) 727-4968