- Technology Readiness Level: 3
Researchers at Purdue University developed a body-mounted system for detecting animal brain activity with a large imaging depth and dual-region imaging capability. Traditional head-mounted two-photon microscopy (TPM) systems have imaging depth approximately 3 times lower than benchtop TPM and lack multiregional imaging ability. The Purdue researchers’ invention provides images of the first five layers of neurons in the neocortex, which is a similar imaging depth to the TPM gold standard. Also, its dual-region imaging capability allows simultaneous monitoring of multiple brain regions to stimulus response. Additionally, using a weight- and torque-balanced system, the researchers were able to monitor the brain activity of freely moving mice. This technology provides more behavior scenarios to be analyzed than those allowable by traditional head-mounted systems. Finally, along with mice, imaging of the brains of larger animals like rats, cats, and monkeys can be accomplished with this system.
Technology Validation: The imaging depth in the Purdue researchers’ systems is 700 microns, whereas the imaging depth in traditional head-mounted TPM systems is 200-300 microns.
- larger imaging depth than traditional head-mounted systems
- dual-region imaging capability
- detection of brain activity of freely moving animals
- brain monitoring of larger animals than allowable by traditional head-mounted systems
- high resolution images
- monitoring brain activity of animals in real-time
Name: Patrick W Finnerty