EPFL10 Knockout in Rice


In this invention, gene-editing of EPFL10 and STOMAGEN using CRISPR/Cas9 in cv. Nipponbare reduces stomatal density while maintaining photosynthesis and enhancing water conservation in rice, yielding lines with c. 80% and 25% of wild-type stomata, respectively. Rice plants with small reductions in stomatal densities are able to conserve water to similar extents as rice plants with large stomatal density reductions but do not suffer from any concomitant reductions in stomatal conductance, carbon assimilation, or thermoregulation.
Rice production is of paramount importance for global nutrition and will be detrimentally affected by climate change.




This technology produces rice with wild-type physiological capacities of stomatal conductance, carbon assimilation, thermoregulation, and yield while conserving more water than wild-type. These attributes could contribute to improved climate resilience in current and future conditions where water is limiting, and temperatures are increased.

Contact Information:

  • Name: Laleh Shayesteh
  • Title :
  • Department :
  • Email : lalehs@berkeley.edu
  • Phone : 510-642-4537
  • Address :