Electrically Active Magnetic Nanoparticle-based Biosensors

Executive Summary

Harmful microbes are present in various sources including water, food, plants, and through human-to-human transmission. The ability to detect them rapidly at a low cost is important to protecting the health and safety of humans and animals and the security of food chains. MSU researchers have developed novel nanoparticle-based biosensors for rapidly sensing pathogens in complex matrices or media. The biosensors can be used in a variety of application settings without the need for pre-purification, lengthy culturing, and amplification. Devices for determining pathogen content as a function of electrical input are also provided.

Description of Technology

This technology covers a suite of inventions relating to biologically enhanced electrically active magnetic nanoparticles (a.k.a. BEAM). The particles are based on magnetic Fe2O3 nanoparticles encapsulated with a conductive polymer such as polyaniline (PANI). The nanoparticles can be coated with a capture reagent that may include antibodies, carbohydrates, antigens, hormones, oligonucleotides, or polynucleotides. Upon mixing with the media containing the bacteria, the coated BEAMs bind to the microbes which can be selectively separated with a magnet to concentrate them. The concentrated nanoparticle-microbes can then be applied to an electrode-based test kit (including screen-printed carbon electrodes) where conductance or resistance can be measured. The nanoparticle-microbe concentrate can also be reacted with a label such as an enzyme, chromogenic substrate, chromophore, chemiluminescent molecules, etc. for alternative measurement approaches. Patents include systems and methods of detection of analytes.

Benefits

  • Simple method of detecting bacteria, viruses, and other pathogens present in a sample
  • Can detect multiple pathogens or specific pathogen
  • Can be used on multiple media such as sputum, water, food matrixes, urine, blood
  • Eliminates pre-enrichment, purification, pre-treatment steps
  • Rapid detection within 1-2 hours
  • Inexpensive test method

Applications

  • Disease and health monitoring
  • Food pathogen detection and monitoring of food quality
  • Environmental water monitoring
  • Agricultural disease detection (crops, plants, farm animal products and health)

Patent Status

Issued US patents US 8,287,810US 8,859,297US 8,936,946US 9,448,236US 9,488,650US 10,739,337

Licensing Rights

Full licensing rights available

References

Biosystems Engineering 2008 paper

Biosensors and Bioelectronics 2009 paper

Biosensors and Bioelectronics 2011 paper

Biosensors 2012 paper

Inventors

Dr. Evangelyn Alocilja, Dr. Sudeshna Pal, Emma Setterington, Dr. Barbara Cloutier, Dr. Michael Anderson

TECH ID

TEC2007-0036, TEC2007-0157, TEC2010-0030, TEC2012-0006

Website

http://msut.technologypublisher.com/technology/48537

Contact Information

TTO Home Page: http://msut.technologypublisher.com

Name: Jon Debling

Title: Technology Manager

Department: MSU Technologies

Email: deblingj@msu.edu