Design and Application of Novel Ligand-Induced Split-Protein Systems

This invention is a split protein system that functions as a biotechnological chemically-induced dimerization (CID) tool.  These CID systems are useful for biological research to control numerous outputs in living organisms such as inducing the activation of a specific protein, protein localization, and inducing transcription.  This novel CID system can be used to stabilize a newly designed ternary complex.

Background

Chemically Induced Dimerization (CID) is a biotechnological method where two or more proteins can bind each other and form a ternary or higher complex only in the presence of a specific small molecule or other dimerizing ligand. These CID systems are used in biological research to control numerous outputs in living organisms such as inducing the activation of a specific protein, protein localization, and inducing transcription. These different ligands in CID systems can be engineered to induce activation of protein outcomes that can be used to control a variety of signaling pathways and their related physiological outputs and thus be used to study biology and treat diseases. The present invention features a novel approach for the engineering of new CID systems based on the recognition that ligand binding may stabilize a newly designed ternary complex. 

Applications

  • Study activation of proteins for controlling signaling pathways and outcomes.
  • Develop novel therapeutics based on protein activation and control of signaling pathways

Advantages

  • New tool for designing and building ternary complexes for targeted protein activation.
  • New tool for designing and building protein:ligand complexes for protein engineering technology
  • Builds on split luciferase assay technology

Website

https://arizona.technologypublisher.com/tech/Design_and_Application_of_Novel_Ligand-Induced_Split-Protein_Systems

Contact Information

TTO Home Page: https://arizona.technologypublisher.com

Name: Laura Silva

Title: Sr. Licensing Manager, COS

Department: TLA

Email: lauras@tla.arizona.edu