Chimeric TLR9/TLR4 Reporter Cell Line

Abstract

This is a cell line that expresses a chimeric protein consisting of the extracellular domain of Toll-like receptor 9 (TLR9) fused to the transmembrane and cytosolic domains of TLR4 (hereafter referred to as TLR9N4C. TLRs are innate immune receptors that respond to microbial ligands and in some cases self-ligands. Responses to self- ligands can lead to autoimmune or autoinflammatory diseases.

Website

https://techtransfer.universityofcalifornia.edu/NCD/24287.html?utm_source=AUTMGTP&utm_medium=webpage&utm_term=ncdid_24287&utm_campaign=TechWebsites

Advantages

The development of inhibitors of certain TLRs has been a goal of the pharmaceutical industry. A primary application of this TLR9N4C-expressing cell line is to screen for inhibitors of TLR9.  UCB is willing to license this cell line to screen for inhibitors of TLR9 activation. 

Potential Applications

  • Screening for compounds that impact activation of TLR9 (activators or inhibitors)
  • Determination of mechanism of action of said compounds (chimeric receptor separates ligand binding from intracellular signaling.
  • Detection of TLR9 ligands in samples 

Contact Information

Name: Craig Kennedy

Email: craig.kennedy@berkeley.edu