Chemically Modified Nell-1 and Methods of Making and Using the Same

UCLA researchers PEGylated NELL-1 by random conjugation using 3 different PEG sizes. The thermal stability, cytotoxicity, in vivo bioactivity and pharmacokinetic behavior of the multiple forms of PEGylated NELL-1 were evaluated and the most effective f…

UCLA researchers PEGylated NELL-1 by random conjugation using 3 different PEG sizes. The thermal stability, cytotoxicity, in vivo bioactivity, and pharmacokinetic behavior of the multiple forms of PEGylated NELL-1 were evaluated and the most effective forms were selected. Specific forms of PEGylation of NELL-1 significantly increase the elimination half-life of the protein from 5.5h to 15.5h while distributing more than 2-3 times the amount of protein to bone tissue in vivo. Systemic NELL-PEG therapy administered every 4-7 days significantly increases not only percent bone volume but also new bone formation throughout the overall skeleton after 4 weeks of treatment.

Abstract

UCLA researchers have developed ways to chemically modify NELL-1, an osteoinductive factor, that significantly prolong the in vivo circulation time of the protein and retain its osteoblastic activity without any appreciable cytotoxicity.

Website

https://techtransfer.universityofcalifornia.edu/NCD/28738.html?utm_source=AUTMGTP&utm_medium=webpage&utm_term=ncdid_28738&utm_campaign=TechWebsites

Advantages

  • This invention allows further development of NELL-1 into an effective systemic therapy for the treatment of osteoporosis. NELL-1 can be administrated with a low dose and low frequency, possibly leading to lower treatment costs and higher patient compliance.  
  • Unlike BMP-2 which has undesirable side effects such as excessive and ectopic bone formation, bone resorption, etc., NELL-1 has fewer side effects occurring during application in vivo.

Potential Applications

A principal application of this invention is for the treatment of osteoporosis.

Contact Information

Name: UCLA Technology Development Group

Email: ncd@tdg.ucla.edu

Phone: 310.794.0558