An Inexpensive and Sustainable Urease Inhibitor to Improve Fertilizer Efficiency

  • Technology Readiness Level: 4

Researchers at Purdue University have developed a urease inhibitor that improves the efficiency of fertilizers. Urease is an enzyme commonly found in plants, fungi, and bacteria that converts urea-based nitrogen fertilizer into ammonia and carbon dioxide. However, ammonia volatilizes to the atmosphere and most of the nitrogen absorbed by plants is in form of nitrate. Therefore, urease inhibitors can improve fertilizer efficiency. The Purdue researchers’ urease inhibitor traps ammonia at a depth of ~5-10 cm and mineralizes it as plant-usable forms. Unlike the industry-standard urease inhibitor (NBPT), the Purdue researchers’ inhibitor is inexpensive, natural (derived from walnut and pecan trees), and eco-friendly. This technology can benefit farmers and any agricultural operations by increasing fertilizer efficiency, reducing costs, along with reducing environmental problems caused by excess of fertilizers.

Technology Validation: A 100 microliter assay containing 50 microliters of extract from green walnut husks inhibited ~70% of urease activity.

Contact Information

Name: Abhijit A Karve


Phone: 765-588-3487