A Device For Continuous Focusing And Rotation Of Biological Cells And Its Application For High Throughput Electrorotation Flow Cytometer

Professor Chiou and coworkers have developed a novel microfluidic device for high throughput analysis of cells using electrorotation (ROT). Using dielectrophoresis (DEP), cells are aligned and focused within a tunnel-shaped electric field. A second set of electrical signals is then applied to rotate the focused cells and a high-speed camera is used to image the rotating cells. Post-processing analysis enables the measurement of cell size, texture, shape, and rotation speed. This technique does not require fluorescent labeling and flow throughput in the device is improved by 4 orders of magnitude compared to conventional electrorotation-based cell analysis, up to 42 cells/sec.

Abstract

UCLA researchers in the Department of Mechanical and Aerospace Engineering have developed a novel device for high-throughput label-free analysis of cells.

Website

https://techtransfer.universityofcalifornia.edu/NCD/29710.html?utm_source=AUTMGTP&utm_medium=webpage&utm_term=ncdid_29710&utm_campaign=TechWebsites

Advantages

  • Analysis of a high concentration of cells (106/mL) 
  • Can be used in flow mode 
  • Four orders of magnitude faster throughput than conventional label-free electrorotation-based cell analysis (42 cells/sec) 
  • Sort and analyze subpopulations of cells

Potential Applications

  • Diagnosis based on cell dielectric properties 
  • Sorting subpopulations of cells 
  • 3D cell imaging and cell growth

Contact Information

Name: UCLA Technology Development Group

Email: ncd@tdg.ucla.edu

Phone: 310.794.0558