7104 – Synthetic Ex Vivo 3D Immune Organoids for B-Cell Culture & Production

A functional, synthetic immune organ (B cell follicle organoid) made of nanocomposite biomaterials100-fold higher and more rapid differentiation of naïve primary B cells to the GC phenotype with robust antibody class switchingThe system does not requi…
  • A functional, synthetic immune organ (B cell follicle organoid) made of nanocomposite biomaterials
  • 100-fold higher and more rapid differentiation of naïve primary B cells to the GC phenotype with robust antibody class switching
  • The system does not require in vivo implantation

Abstract:

Invention Summary

A synthetically engineered immune organoid for growth, proliferation, activation, differentiation, and immunoglobulin switching of primary naïve B cells in a three dimensional structure.

Technology Overview

Secondary immune organs, such as lymph node, tonsil, and spleen, are highly structured tissues which dynamically change mechanical and biological functionality in response to antigens
Of particular importance is the activation of naïve B cells to form sub-anatomical structures that program B cell conversion into antibody producing cells, a powerful defense mechanism against pathogens
Currently, researchers rely heavily on live animal models to understand immune cell development, functioning, and screening of immunotherapies against diseases, but such approaches are costly with long turnaround times
Cornell researchers have created a functional, synthetic immune organ (B cell follicle organoid) made of nanocomposite biomaterials, which recapitulates the anatomical microenvironment of a lymphoid tissue. It is comprised in part, of a RGD-presenting hydrogel system, reinforced with synthetic nanoparticles, the stiffness of which mimics the native secondary lymphoid tissue
Compared to 2D co-cultures, this novel 3D immune organoid resulted in 100-fold higher and more rapid differentiation of naïve primary B cells to the GC phenotype with robust antibody class switching

Website:

https://cornell.flintbox.com/technologies/2DDC4DE4D15548D79E99B25BC9B98C47

Advantages:

  • Development and differentiation of primary naïve B cells into germinal centers (GC) phenotype
  • Accelerated GC reaction ex vivo
  • Precise control of the magnitude and rate of GC reaction
  • Robust antibody class switching
  • The system does not require in vivo implantation

Potential Applications:

  • High-affinity monoclonal antibody production against specific antigens ex vivo
  • Screening of biotherapeutic compounds which target B cells
  • Implant immune organoid to elicit an in vivo response
  • Research tool for real-time study of germinal center B cell physiology and pathology

Contact Information:

Name: Phillip Owh

Title :

Department :

Email : po62@cornell.edu

Phone : 1-607-254-4508

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