2451 – RNA-activated CRISPR system for cleaving DNA and RNA molecules

Unique CRISPR system, Type III-B or Cmr complex. Can cleave DNA or RNA. Does not require a PAM sequence for targeting. RNA-only guided cleavage.
  • Unique CRISPR system, Type III-B or Cmr complex.
  • Can cleave DNA or RNA.
  • Does not require a PAM sequence for targeting. RNA-only guided cleavage.

Abstract

This technology is a CRISPR-RNA (crRNA) and a Cmr complex. The crRNA is single-stranded with a tag region (5-12 nucleotides) and a guide region (at least 15 nucleotides). The Cmr complex is at least four proteins (Cmr 2, Cmr 3, Cmr 4, and Cmr 5), wherein Cmr 4 contains a mutation to reduce RNase activity of the complex. A genetically modified cell is available to produce the crRNA and Cmr complex.

Established methods of use are also provided.

  1. Method for determining whether a predetermined RNA is present or absent in a sample.
  2. Method for detecting the presence or absence of cleavage of a substrate DNA.
  3. Method for cleaving a predetermined RNA molecule.

Potential Applications

Genetic modification systems are key for certain advancements in the medical and agricultural fields. While still in early phase development, gene editing technologies could be applied to treating genetic disorders such as cystic fibrosis, sickle cell anemia, marfan syndrome, Huntington’s disease, etc. Genetically modified crops are already produced around the world constituting an $18 billion business annually. Similarly, this technology can be applied to bacterial systems for various research/production needs.