2022-135 – Transformation-Mediated Intracellular Chromosome Synthesis

  • Replace whole chromosomes, and assemble chromosomes into new chromosome architectures
  • Combination of genetic material from different individuals into chimeric chromosomes
  • Multiplex deletion of specific genes in a single transformation

Abstract

Our R&D team developed a method to capture segments of natural chromosomes and to then reassemble them into new chromosomes inside living eukaryotic cells. This method can be used to replace whole chromosomes and allows multiplex deletion of specific genes in a single transformation. Synthetic chromosomes can be programmed to assemble into new chromosome architectures. A further application is the combination of genetic material from different individuals or species into chimeric chromosomes.

Advantages

  • Efficient gene editing method to achieve several deletions, insertions or chromosome assemblies in a single transformation.
  • Replacement of whole chromosomes in single steps.
  • Combination of genetic material from different individuals or species into chimeric chromosomes.

Contact Information

Name: Stefan Schweizer

Email: stefans@usc.edu

Phone: (213) 821-6067