Hybridoma cell lines were generated by fusion of P3X63-Ag8.653 mouse myeloma cells with spleen cells from BALB mice immunized with GST-Lgl-1 fusion protein. Monoclonal antibodies against Lgl-1 were obtained from this cell line. These antibodies recognize mouse, huma, rat, and dog Llg1, but do not recognize its close paralog Llgl2 from mouse or humans. The mouse monoclonal IgG antibody can recognize Llg1 by ELISA, Immunoblot, Immunoprecipitation, and by Immunofluorescence of fixed polarized cells.
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