Cell-free selection of site-specific polypeptide binders via tethering

IP Status: US Patent Issued #10,724,076 Applications Protein based biologics Drug discovery screening Technology Overview A new method for isolating and identifying biomolecules that bind to a specific target epitope. This novel techno…

Applications

  • Protein-based biologics
  • Drug discovery screening

Technology Overview

A new method for isolating and identifying biomolecules that bind to a specific target epitope. This novel technology combines cell-free display, engineered protein scaffold libraries, and disulfide tethering methods into one optimized protocol that selectively enriches for polypeptides that are target-site specific. This technology can be used to identify polypeptide therapeutics for treating a broad range of diseases including cancer, where it is critical to ensure therapeutics target cancerous mutations without affecting normal proteins.

Phase of Development
TRL: 1-3
The research is at a conceptual stage with some supporting in vitro data. Proof of concept demonstrating the technique can be used to perform a bulk enrichment of the peptide library against a test protein has been obtained.


Desired Partnerships
This technology is now available for:

  • License
  • Sponsored research
  • Co-development
  • Please contact our office to share your business’ needs and learn more.

Researchers
Casim Sarkar, Ph.D. Associate Professor, Department of Biomedical Engineering

Abstract

In vitro selection method for the engineering of protein-based high-affinity binders to targets of interest with a potential pharmacological value

Website

https://license.umn.edu/product/cell-free-selection-of-site-specific-polypeptide-binders-via-tethering

Contact Information

Name: Rayla Vilar

Email: pinto115@umn.edu

Phone: 5158173606