9201 – Highly Specific Capture of Target Macromolecules Using Aptamers for Compositional and Structural Analysis

A method to use specially designed aptamers to capture target macromolecules from crude samplesHigh affinity and specificity to target macromolecules to simplify downstream analysisCost efficient to produce and applicable to a wide variety of macromole…
  • A method to use specially designed aptamers to capture target macromolecules from crude samples
  • High affinity and specificity to target macromolecules to simplify downstream analysis
  • Cost efficient to produce and applicable to a wide variety of macromolecules including proteins, lipids, nucleotides, sugars, etc.

Abstract:

Invention Summary

A method of using aptamers to capture target macromolecules with high affinity and specificity, simplifying downstream analytical procedures, including mass spectrometry, cryo-electron microscopy, and nucleotide sequencing.

Technology Overview

Macromolecules play a vital role in nearly all cellular functions and understanding changes in their quantity and intermolecular interactions in biological processes is crucial for basic research and clinical applications such as drug development.
Selective enrichment of macromolecules from crude bio-samples often relies on antibody-based methods and while the target macromolecules are found in the processed sample, there are often also contaminants from the enrichment procedure, resulting in suboptimal outcomes of downstream analytical procedures.
This invention uses specially designed aptamers to capture target macromolecules from a crude sample with high specificity and high affinity.
Aptamer-assisted target enrichment simplifies downstream analytical procedures.
Aptamers are cost-efficient to synthesize and can be used to capture a wide variety of macromolecules, including proteins, lipids, nucleotides, sugars, etc.
Cornell researchers have successfully applied this method to enrich target macromolecules for mass spectrometry, cryo-electron microscopy, and nucleotide sequencing either on its own or in sequential combination with other purification procedures such as immunoprecipitation.

Website:

https://cornell.flintbox.com/technologies/CCC66759B2524F9EB2680ADC500D931B

Advantages:

  • High specificity and affinity and applicable to a wide range of macromolecules, proteins, lipids, nucleotides, sugars, etc.
  • Efficient enrichment of target macromolecules and enhanced sensitivity and accuracy of downstream compositional and structural analysis.
  • Cost-efficient

Potential Applications:

  • Target enrichment for analyses (e.g., mass spectrometry, cryo-electron microscopy, nucleotide sequencing, etc.)
  • Affinity purification of target macromolecules (e.g., peptide synthesis, antibody production, etc.)

Contact Information:

Name: Phillip Owh

Email: po62@cornell.edu

Phone: 1-607-254-4508