2021-113 – Ckb.Flag mouse

CKB expression is controlled under endogenous genomic regulatory elements, so that CKB-Flag is expressed at physiologically-relevant levels. Commercially available CKB antibodies do not work for immunoprecipitation, and are not 100% specific. The Ckb.F…
  • CKB expression is controlled under endogenous genomic regulatory elements, so that CKB-Flag is expressed at physiologically-relevant levels.
  • Commercially available CKB antibodies do not work for immunoprecipitation, and are not 100% specific. The Ckb.Flag mouse bypasses this technical hurdle and allows investigators to leverage the specificity of FLAG affinity purification to isolate the major
  • Given the specificity of affinity purification, post-translational modifications can be identified for the first time ever, from any tissue, under physiological interventions, such as different diets, ambient temperatures, physiological stress (exercise)

Website:

https://mcgill.flintbox.com/technologies/1DB46BD1540F47D0A222CEC73AC2BB22

Advantages:

Compared to existing technologies, CKB can be isolated from any tissue in mice in response to any physiologically relevant state.

Potential Applications:

This mouse model will be a valuable resource for researchers wishing to study creatine metabolism, in any physiological or pathological state.

Contact Information:

Name: Mostafa Ghozlan

Title :

Department :

Email: mostafa.ghozlan@mcgill.ca

Phone: 514-398-5887

Address :