- CKB expression is controlled under endogenous genomic regulatory elements, so that CKB-Flag is expressed at physiologically-relevant levels.
- Commercially available CKB antibodies do not work for immunoprecipitation, and are not 100% specific. The Ckb.Flag mouse bypasses this technical hurdle and allows investigators to leverage the specificity of FLAG affinity purification to isolate the major
- Given the specificity of affinity purification, post-translational modifications can be identified for the first time ever, from any tissue, under physiological interventions, such as different diets, ambient temperatures, physiological stress (exercise)
Compared to existing technologies, CKB can be isolated from any tissue in mice in response to any physiologically relevant state.
This mouse model will be a valuable resource for researchers wishing to study creatine metabolism, in any physiological or pathological state.
Name: Mostafa Ghozlan